Selection of carrier for immobilization of fructosyltransferase from Aureobasidium pullulans
Z. Platková, M. Polakovič, V. Štefuca, M. Vandáková, and M. Antošová
Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia
Received: 27 July 2006 Accepted: 7 September 2006
Abstract: The study deals with the development of an immobilized biocatalyst for the production of fructooligosaccharides. Several commercial anion-exchange resins and polymethacrylate carriers were tested for the immobilization either by the direct attachment of fructosyltransferase or by the attachment accompanied by simultaneous glutaraldehyde crosslinking in the pH range from 5.7 to 7.1. On the basis of the efficiency of immobilization and the storage stability, Amberlite IRA 900 and Dowex Marathon MSA were selected as the best carriers for fructosyltransferase immobilization by direct attachment at pH 5.7.
Keywords: fructosyltransferase - immobilization - Aureobasidium pullulans - anion-exchange resins - polymethacrylate carriers - glutaraldehyde crosslinking
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Chemical Papers 60 (6) 469–472 (2006)