ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Detection of colistin (Polymyxin B) a restricted drug in chicken eggs using a label-free immunosensor based on Au screen-printed electrodes

Sonali Khanal, Akriti Taneja, Harsh Kumar, Rachna Verma, Kamil Kuca, and Dinesh Kumar

School of Bioengineering and Food Technology, Shoolini University of Biotechnology and Management Sciences, Solan, India

E-mail: dkchatanta@gmail.com

Received: 6 May 2023  Accepted: 6 October 2023

Abstract:

This article highlights a label-free point-of-care detection of colistin (Polymyxin B) with modified immunosensor in chicken eggs. The manufacturing of screen-printed gold electrodes (Au-SPEs) is claimed to improve the sensor's sensitivity and point-of-care diagnostics, outperforming the outputs in terms of time and accuracy. The sensor attached a specific colistin antibody to a conventional Au-SPE that had been polyethyleneimine (PEI) modified by a carbodiimide-mediated amide coupling procedure. Using cyclic voltammetry (CV) and (K₃Fe (CN)₆)⁻ as a redox probe during each stage of fabrication, this immunosensor captured the electrochemical changes in the sample laced with colistin. Characterization of Au-SPEs was done with AFM for surface morphology and topographic data and FTIR for the functional group confirmation. Limit of Detection (LOD) of 1.539 µg/kg of egg with high specificity toward antigen against other antibiotics was observed. Therefore, the proposed immunosensor based on Au-SPEs has potential applicability for early detection of colistin which could be a promising tool for its rapid detection.

Keywords: Colistin; Chicken eggs; Antibody; Immunosensors; Biosensors

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-023-03138-y

Chemical Papers 78 (2) 1009–1020 (2024)