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Mesophilic and thermophilic dark fermentation course analysis using sensor matrices and chromatographic techniques

Edyta Słupek, Patrycja Makoś, Karolina Kucharska, and Jacek Gębicki

Department of Process Engineering and Chemical Technology, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland



Received: 30 June 2019  Accepted: 23 November 2019


Production of biofuels from biomass is expected to benefit the society and the environment. At present, bio waste residues processing includes hydrolysis, dark fermentation, photofermentation, pyrolysis, gasification, and chemical synthesis. As the composition and the chemical structure of organic substances affect the efficiency of mentioned processes, it is believed that the glucose concentration is a crucial parameter for the evaluation of the efficiency of biological processes. Also, the control of by-products formulated during each stage of biomass processing affects the course of dark fermentation. Therefore, model processes regarding mesophilic and thermophilic dark fermentation were carried out. Glucose as a sole carbon source was applied as the fermentation broth and Faloye-pretreated activated municipal wastewater sludge was introduced as the source of sporulating microorganisms. Production of hydrogen and methane was controlled by means of sensor matrices. Obtained results are comparable to those obtained using the standard method based on gas chromatography and indicate the suitability of their application for online routine analyses of hydrogen and methane during fermentation processes. In addition, the fermentation broth was also examined by means of gas and liquid chromatography in the scope of glucose reduction, and generation of volatile fatty acids and phenols.

Keywords: Biogas analysis; Chromatographic analysis; Hydrogen; Methane; Dark fermentation; Sensor matrices

Full paper is available at

DOI: 10.1007/s11696-019-01010-6


Chemical Papers 74 (5) 1573–1582 (2020)

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