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Establishment of a HPLC fingerprint method and its application in evaluating the overall change of organic matter in a complex environment: taking the settled house dust as an example

Qin Yang, Handong He, Tianyi Luo, Zijie Wang, Xiaofang Zhang, and Jianghong Yang

College of Civil Engineering, Guizhou University, Guiyang, China



Received: 24 June 2019  Accepted: 18 November 2019


Organic contamination has been recognized as a matter of concern, and simultaneous monitoring of various organic contaminants in the complex environment has always been a part of the efforts of scientists. In the present study, settled house dust (SHD) was selected to establish HPLC fingerprint method for simultaneously evaluating organic matters in an indoor environment. The relative standard deviations (RSDs) of the relative retention time (RRT) and relative peak area (RPA) for precision were 2.46% and 0.47%, respectively, while the RSDs of RRT and RPA for repeatability were 2.51% and 0.50%, respectively, and for stability within 24 h were 2.51% and 0.49%, respectively, which indicated good reliability of the method. Then, the method was used to evaluate the trend of indoor organic matters of Yunyan district (urban) and Gangou village (rural) from 2012 to 2014. The results showed the overall organic matters of Yunyan did not change significantly within 2 years, while the organic matters of Gangou village were a little aggravated by the appearance of some suspicious matters. Our study suggests a method with the capability of simultaneously evaluating various organic matters and a possibility for detecting those that are not currently on the list of priority organic contaminants.

Keywords: Indoor environment; Organic matters; HPLC; Fingerprint; Overall evaluation

Full paper is available at

DOI: 10.1007/s11696-019-01005-3


Chemical Papers 74 (5) 1551–1559 (2020)

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