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Nitrogen-doped reduced graphene oxide as a sensing platform for detection of guanine and application in cell necrosis

Guangjie Song, Rongjin Zhang, Xuechan Jiang, Fuxin Liu, and Xiuhui Liu

Northwest Normal University, Lanzhou, People’s Republic of China



Abstract: In this paper, a novel electrochemical sensor was fabricated based on nitrogen-doped reduced graphene oxide (N-RGO). The morphology, structure and electrochemical properties of N-RGO were investigated by transmission electron microscopy (TEM), atomic force microscope (AFM), X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry (CV). AFM indicated the thickness of N-RGO is about 1.0 nm. The electrochemical experiments demonstrated that N-RGO possesses a relatively large surface area, strong adsorptive ability and excellent electrical conductivity. It was believed that N-RGO is a promising candidate for applications in electrochemical sensors and biosensors. Moreover, N-RGO modified glassy carbon electrode (N-RGO/GCE) exhibited good electrochemical response toward the oxidation of guanine with a linear range covering 4.14 × 10−7–3.71 × 10−4 M, and the corresponding detection limit (LOD) of 1.38 × 10−7 M. Eventually, the proposed sensor could be used to monitor cell necrosis by means of detecting the increase of the current response of guanine. Thus, the work is very meaningful in the field of biological cytology and pathology. A sensitive sensor for guanine detection is constructed based on nitrogen-doped reduced graphene oxide (N-RGO), which was synthesized by a facile method. This paper is the first time to monitor cell necrosis by detecting the increase of guanine content.

Keywords: Nitrogen ; Doped reduced graphene oxide ; Cell contents ; Guanine ; Cell necrosis ; Cyclic voltammetry 

Full paper is available at

DOI: 10.1007/s11696-019-00856-0


Chemical Papers 74 (1) 89–98 (2020)

Monday, July 15, 2024

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