ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7

Published monthly
 

Estimation of plasmid concentration in batch culture of Escherichia coli DH5α via simple state observer

Fernando Grijalva-Hernández, V. Peña Caballero, Pablo A. López-Pérez, and Ricardo Aguilar-López

CINVESTAV-IPN, Mexico City, CDMX, Mexico

 

E-mail: raguilar@cinvestav.mx

Abstract: The aim of this paper is to present an alternative state observer structure for online estimation purposes of the key dynamical variables in a class of batch culture for plasmid production; the latter has been extremely attractive to be used as DNA vaccines or gene therapy. A mathematical model for culture of Escherichia coli DH5α-harboring plasmid was considered a benchmark system for the application of the proposed estimation methodology. Local observability analysis revealed that the system is partially observable for plasmid concentration considering only the biomass concentration in the batch culture as the measured variable. The proposed observer is designed with a simple proportional–integral feedback of the measured biomass concentration, where under the proposed design, the observer gain´s array can compensate the main nonlinearities of the estimation error dynamics. The convergence of estimated variables to the real ones is mathematically analyzed, reaching an asymptotic behavior. Numerical experiments were performed, where a comparison with a standard extended Luenberger observer was done and the proposed estimation methodology revealed a satisfactory performance.

Keywords: Plasmid concentration monitoring ; Batch bioreactor ; PI-type observer ; Mathematical analysis 

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-018-0478-7

 

Chemical Papers 72 (10) 2589–2598 (2018)

Friday, March 29, 2024

IMPACT FACTOR 2021
2.146
SCImago Journal Rank 2021
0.365
SEARCH
Advanced
VOLUMES
European Symposium on Analytical Spectrometry ESAS 2022
© 2024 Chemical Papers