ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Study of the binding sites in the biomass of Aspergillus niger wild-type strains by FTIR spectroscopy
Katarína Gáplovská, Alexandra Šimonovičová, Radoslav Halko, Lenka Okenicová, Mária Žemberyová, Slavomír Čerňanský, Paula Brandeburová, and Tomáš Mackuľak
Comenius University, Bratislava, Slovakia
Abstract: Fourier transform infrared (FTIR) spectroscopy studies were performed to confirm and to provide information on the identity and binding characteristics of the chemical groups responsible for the binding of elements using Aspergillus niger (A. niger) wild-type strains. Two absorption bands in the 3690–3615 and 2970–2895 cm−1 regions can characterize stretching vibrations OH and CH groups in fatty acids, respectively, and intensive bands around of 1600 cm−1 and by 1048 cm−1 correspond to stretching vibrations of C=O groups of amides (amide I) or stretching vibrations ν(C–N). The FTIR results confirmed that no extra differences between IR spectra of A. niger in raw biomass and in solid rest after extraction with chloroform were observed. The small differences were observed in IR spectra of A. niger in chloroform after extraction.
Keywords: Aspergillus niger ; Bioaccumulation ; Binding sites ; FTIR spectroscopy ; UV spectroscopy ; Elemental analysis
Full paper is available at www.springerlink.com.
Chemical Papers 72 (9) 2283–2288 (2018)