ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7

Published monthly

Is it possible to use the DPPH and ABTS methods for reliable estimation of antioxidant power of colored compounds?

Małgorzata Olszowy and Andrzej L. Dawidowicz

Maria Curie Sklodowska University, Lublin, Poland



Abstract: Negative impact of radicals on humans and animals is responsible for growing research interest in antioxidant properties of substances, which protect living organisms from the damaging influence of these reactive species. Among numerous methods for antioxidant activity estimation, DPPH and ABTS are the most popular and commonly used ones due to their ease, speed, sensitivity and the usage of stabile radicals. The paper shows and discusses the antioxidant properties of a few compounds exhibiting different extinction of the wavelength used to monitor colored radical depletion in spectrophotometric measurements—e.g. 516 and 744 nm in DPPH and ABTS assays, respectively. As results from the presented data, the difference between true and spectrophotometrically estimated IC50 values for colored antioxidants depends not only on extinction coefficient of the monitored wavelength by the antioxidant but also on its remaining concentration in the measuring system. The presented results seems to be helpful for the researches examining coloured antioxidants and plant extracts containing substances having spectra in Vis region.

Keywords: DPPH method ; ABTS method ; Coloured antioxidants ; IC50 determination 

Full paper is available at

DOI: 10.1007/s11696-017-0288-3


Chemical Papers 72 (2) 393–400 (2018)

Monday, September 26, 2022

SCImago Journal Rank 2021
European Symposium on Analytical Spectrometry ESAS 2022
© 2022 Chemical Papers