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A label-free electrochemical immunosensor based on multi-functionalized graphene oxide for ultrasensitive detection of microcystin-LR

YunXia Hu, Fang Shang, Yuan Liu, Sui Wang, Yufang Hu, and Zhiyong Guo

Ningbo University, Ningbo, People’s Republic of China

 

E-mail: wangsui311@163.com

Abstract: In this paper, a label-free electrochemical immunosensor for ultrasensitive detection of microcystin-leucine-arginine (MC-LR) based on multi-functionalized graphene oxide was constructed. The graphene oxide has a large surface area for the immobilization of the antibody. Meanwhile, the introduction of the AuNPs and 1-butyl-3-methylimidazolium hexafluorophosphate could enhance the response of the current by improving the electrical conductivity. Thus the electrochemical immunosensor could be prepared through a one-step process and differential pulse voltammetry was employed to detect sensitively MC-LR. Under optimal conditions, the current response of the immunosensor decreased proportionally to the logarithmic concentrations of MC-LR in the range of 0.1–1000 ng/mL with a detection limit of 0.1 ng/mL (S/N = 3). This one-step label-free electrochemical immunosensor showed good performance in specificity, stability, reproducibility, and application.

Keywords: Microcystin-leucine-arginine ; Electrochemical ; Immunosensor ; Graphene oxide ; Differential pulse voltammetry (DPV) 

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-017-0258-9

 

Chemical Papers 72 (1) 71–79 (2018)

Monday, October 07, 2024

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