ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7

Published monthly
 

Analysis of spectinomycin in fermentation broth by reversed-phase chromatography

Hong De Yan, Pei Ya Xu, Hai Chan Huang, and Juan Ping Qiu

College of Biological and Environmental Engineering, Zhejiang University of Technology, 310014 Hangzhou, China

 

E-mail: qiujping@zjut.edu.cn

Received: 18 January 2009  Revised: 16 March 2009  Accepted: 17 March 2009

Abstract: A pre-column derivatized high-performance liquid chromatographic (HPLC) method with ultraviolet-visible detection was developed to measure the concentrations of spectinomycin in fermentation broth. Derivatization reagents, 2,4-dinitrophenylhydrazine in acetonitrile (5 mg mL−1) and trifluoroacetic acid in acetonitrile (0.8 mol L−1), were added to an aliquot of the fermentation broth, and the mixture was incubated for 60 min at 70°C. The resulting derivative was separated from other compounds by isocratic elution in a reversed-phase column Zorbax SB-C18 (250 mm × 4.6 mm, 5 µm). Mobile phase consisted of acetonitrile, tetrahydrofuran, and water (φ r = 40: 35: 25) and the flow rate was 1.0 mL min−1. The detection wavelength was 415 nm. The standard curve for spectinomycin sulfate was linear with correlation coefficients of 0.9997 in the range of 25 µg mL−1 to 600 µg mL−1. The relative standard deviation values ranged from 0.43 % to 2.18 % depending on the concentration of samples. The average recovery was 101.5 %. The limit of detection was 50 ng mL−1.

Keywords: spectinomycin - derivatization - analysis - separation - HPLC - fermentation broth

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-009-0064-0

 

Chemical Papers 63 (6) 635–640 (2009)

Friday, July 19, 2019

IMPACT FACTOR 2018
1.246
SCImago Journal Rank 2018
0.274
SEARCH
Advanced
VOLUMES
International Conference on Coordination and Bioinorganic Chemistry
46th International Conference of SSCHE
© 2019 Chemical Papers