ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Chemiluminescence mechanisms of cerium-norfloxacin and its application in urine analysis
Lijun Wei, Xianglei Cheng, Jin-Ming Lin, Hongping Cai, and Fengqi Huang
School of Public Health, Nanchang University, Nanchang, 330006, China
Received: 30 July 2008 Revised: 4 October 2008 Accepted: 7 October 2008
Abstract: In this article, novel chemiluminescence mechanisms between norfloxacin and cerium(IV) in an acidic medium were studied. Chemiluminescence
spectra of the present system were recorded observing three maximum emissions at about 475 nm, 550 nm, and 620 nm, respectively.
The results indicate that the chemiluminescence peaks located at 475 nm and 620 nm can be ascribed to the emission of a singlet
oxygen, while the chemiluminescence emission at 550 nm occurred in course of the reaction between acidic cerium(IV) and the
phenolic intermediate. Under optimum conditions, the chemiluminescence intensity was linear with the concentration of norfloxacin
over the range of 2.0 × 10−8−1.0 × 10−5 g mL−1 and the detection limit of 1.0 × 10−8 g mL−1 (S/N = 3). The relative standard deviation was 1.94 % for a 4.0 × 10−7 g mL−1 norfloxacin solution considering eleven repeated measurements. The present chemiluminescence system was successfully applied
in the determination of norfloxacin in pharmaceutical preparations and concentration-time profiles in urine.
Keywords: norfloxacin - cerium - chemiluminescence - mechanisms - urine
Full paper is available at www.springerlink.com.
Chemical Papers 63 (3) 358–365 (2009)