ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Application of isotachophoretic and conductometric methods for neomycin trisulphate determination
Marzanna Kurzawa, Aneta Jastrzębska, and Edward Szłyk
Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Toruń, 7 Gagarin str., Poland
Received: 18 January 2008 Revised: 3 September 2008 Accepted: 4 September 2008
Abstract: Determination of neomycin trisulphate (NMS) in a dosage form (Neox and Neosol) was carried out by capillary isotachophoresis
(cITP) with conductometric detection. The following electrolytes: leading: 10 mmol dm−3 sodium acetate + 0.08 % hydroxyethylcelulose (HEC) and acetic acid to pH = 5.5, and terminating: 10 mmol dm−3
β-alanine were tested for isotachophoretic separation of NMS. The calibration curve was linear over the range of 10.00 mg dm−3 to 100.00 mg dm−3 with LOD = 5.69 mg dm−3 and LOQ = 18.96 mg dm−3. The results were compared to the conductometric determination of NMS with: ammonium molybdate (VI), silver nitrate (V) and
Reinecke salt. Good accuracy was obtained from conductometric titration of NMS with Reinecke salt, the recoveries being as
follows: 100 % (RSD = 1.99 %); 96.17 % (RSD = 2.10 %) and 95.22 % (RSD = 1.55 %) for NMS in pure form, Neosol and Neox, respectively.
Keywords: capillary isotachophoresis - neomycin trisulphate - Neox - Neosol - conductometric titration
Full paper is available at www.springerlink.com.
Chemical Papers 63 (3) 255–260 (2009)