ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Influence of reaction medium composition on enzymatic synthesis of galactooligosaccharides and lactulose from lactose concentrates prepared from whey permeate
Marek Adamczak, Danuta Charubin, and Włlodzimierz Bednarski
Department of Food Biotechnology, University of Warmia and Mazury, Olsztyn J. Heweliusz St. 1, 10-718 Olsztyn, Poland
Received: 26 June 2008 Revised: 6 October 2008 Accepted: 7 October 2008
Abstract: The paper presents the results of investigations into the technological possibilities of controlling the transgalactosylation
process of lactose in permeate after whey ultrafiltration in order to improve the efficiency of galactooligosaccharides or
lactulose synthesis. The synthesis efficiency was influenced by the selection of a β-galactosidase preparation, substrate
concentration and, in the synthesis of lactulose, also by the ratio of lactose and fructose added to the reaction mixture.
The obtained synthesis efficiency of GOS and, most of all, of lactulose (65 g L−1), may be found satisfactory. The study also resulted in a proposed GOS or lactulose concentrates (concentrated or dried)
production technology using permeate after ultrafiltration of milk or whey as lactose sources.
Keywords: β-d-galactosidase - whey-permeate - galactooligosaccharides - lactulose - transgalactosylation
Full paper is available at www.springerlink.com.
Chemical Papers 63 (2) 111–116 (2009)