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Fructosyltransferases: The Enzymes Catalyzing Production of Fructooligosaccharides

M. Antošová and M. Polakovič

Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, SK-812 37 Bratislava

 

E-mail: antosova@cvt.stuba.sk

Received: 30 March 2001

Abstract: This article reviews the information about fructosyltransferases, the enzymes producing important prebiotic compounds, fructooligosaccharides. The chemical structure, safety and physiological effects, preparation and application of fructooligosaccharides are discussed. Plant and microbial sources of fructosyltransferases are presented. A detailed description is devoted to the mechanisms of action, substrate specificities, and regioselectivities of fructosyltransferases. Their physicochemical and catalytic properties are also discussed. Finally, the methods of purification of fructosyltransferases reported in literature are summarized in respect to the yield and factor of purification.

Full paper in Portable Document Format: 556a350.pdf

 

Chemical Papers 55 (6) 350–358 (2001)

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