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Electrotransformation of Bacteria by Plasmid DNA: Effect of Serial Electroporator Resistor

B. Rittich, K. Manová, A. Španová, and L. Pribyla

Institute of Food Chemistry and Biotechnology, Faculty of Chemistry, Technical University, CZ-637 00 Brno

 

Abstract: Electroporation is a method widely used for the delivery of foreign DNA into plant, animal, and bacterial cell. We studied the influence of serial electroporator resistors (22 Ω, 330 Ω, and 510 Ω) on electroporation parameters (electrotransformation efficiency and frequency). Bacteria of Salmonella typhimurium LB 5000 and DNA of plasmid pUC19 were used as a source of cells and DNA, respectively. The highest transformation frequency (number of transformants/number of survived cells) was achieved with the 22 Ω serial resistor; the highest transformation efficiency (number of transformants/ μg DNA) was achieved with the 330 Ω serial resistor. For the relationship between the number of transformed cells (nt) and the concentration of DNA (ρ) the equation In nt = a - b{ρ} + d In{ρ} (where a, b, d are constants) was used. It was shown that the dependence of nt vs. ρ may be influenced by a serial electroporator resistor, too.

Full paper in Portable Document Format: 504a245.pdf

 

Chemical Papers 50 (4) 245–248 (1996)

Monday, July 22, 2019

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