ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Determination of norfloxacin in pharmaceuticals, human serum, and urine using a luminol—dissolved oxygen chemiluminescence system
X. D. Shao, H. Y. Liu, X. F. Gao, W. Q. Chen, and Z. H. Song
Department of Chemistry, Northwest University, Xi’an, 710069, China
Received: 2 April 2007 Revised: 21 May 2007 Accepted: 29 May 2007
Abstract: A sensitive chemiluminescence method, based on the enhancive effect of norfloxacin on the reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of norfloxacin. The increment of the chemiluminiscence intensity was proportional to the concentration of norfloxacin, giving a calibration graph linear over the concentration from 0.4 ng mL−1 to 400.0 ng mL−1 (r 2 = 0.9988) with the detection limit of 0.1 ng mL−1 (3 × σ noise). At the flow rate of 2.0 mL min−1, a complete determination of norfloxacin, including sampling and washing, could be accomplished in 30 s with the relative standard deviation lower than 3.0 %. The proposed method was applied successfully to determine norfloxacin in pharmaceuticals, human urine, and serum. Possible mechanism of the reaction was also discussed.
Keywords: norfloxacin - chemiluminescence - luminol - serum - urine - flow-injection
Full paper is available at www.springerlink.com.
Chemical Papers 61 (5) 353–358 (2007)