ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7

Published monthly
 

High-Performance Liquid Chromatography Analysis of Thiazolidine-4-carboxylic Acids in Human Serum Samples

J. Y. Al-Ja'afreh, Š. Hatrík, and E. Havránek

Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, SK-832 32 Bratislava

 

Abstract: In the presented paper we studied analytical characteristics of three thiazolidine-4-carboxylic acids. These compounds obtained by condensation of aldehydes with L-cysteine are interesting from the biological point of view. The proposed method involves a solid phase extraction (SPE) of thiazolidinic acids and their precursor aldehydes from human serum using C18 SPE columns. Separation of the six analytes was achieved within 20 min using a reversed-phase HPLC SEPARON SGX C18 column protected by precolumn, with mobile phase of methanol-water (φr = 55 : 45) containing 5 cm3 of acetic acid per 1 dm3 of mobile phase (apparent pH 5.5). The recoveries of all studied compounds from the human serum were in the range of 86-89 %, the detection limits were 30 ng cm-3 of serum.

Full paper in Portable Document Format: 532a89.pdf

 

Chemical Papers 53 (2) 89–92 (1999)

Friday, December 04, 2020

IMPACT FACTOR 2019
1.680
SCImago Journal Rank 2019
0.331
SEARCH
Advanced
VOLUMES
XXVIII. International Conference on Coordination and Bioinorganic Chemistry
© 2020 Chemical Papers