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Optimization of Dextran and Mannan Dialdehydes Preparation and Examination of their Biospecific Interaction with Concanavalin A

J. Masárová, D. Mislovičová, and P. Gemeiner

Institute of Chemistry, Slovak Academy of Sciences, SK-842 38 Bratislava

 

Abstract: The periodate oxidation of dextran and mannan was optimized to prepare activated polysaccharides (containing aldehyde groups) suitable for conjugation with proteins. The mild reaction conditions to achieve available degree of oxidation, while the depolymerization was prevented, were estimated. The products containing up to 16 aldehyde groups per dextran molecule and up to 28 aldehyde groups per mannan molecule were obtained, while a low decrease of Mr of both polysaccharide chains, from 18 400 to 17 000 of dextran and from 55 000 to 49 500 of mannan, respectively, was observed.Using the precipitation method the interaction of Concanavalin A with both. original as well as modified, subsequently reduced, dialdehyde polysaccharides was investigated. Only the precipitation of mannan and its modified form with Concanavalin A was observed. The oxidation of mannan up to 20 aldehyde groups per mannan molecule caused only a very little decrease of affinity of prepared samples to Concanavalin A.

Full paper in Portable Document Format: 552a130.pdf

 

Chemical Papers 55 (2) 130–135 (2001)

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