ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Improved DPPH determination for antioxidant activity spectrophotometric assay
M. R. Szabo, C. Idiţoiu, D. Chambre, and A. X. Lupea
Chemical and Technological Research Centre, Food Engineering, Tourism, and Environmental Protection Faculty, “Aurel Vlaicu” University, Elena Dragoi Street 2, Arad, 310330, Romania
Received: 3 August 2006 Revised: 1 December 2006 Accepted: 4 December 2006
Abstract: An improved procedure for determination of the residual DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical concentration was proposed taking into account the absorbance of both DPPH free radicals and DPPH nonradical (1,1-diphenyl-2-picrylhydrazine) stable form. The calculated residual DPPH free radical concentrations were compared with those obtained from a calibration curve and variation coefficients below 10 % were found.
Keywords: DPPH determination - antioxidant activity - spectrophotometric assay - free radicals
Full paper is available at www.springerlink.com.
Chemical Papers 61 (3) 214–216 (2007)