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Enzyme mimics of Os/Ag nanoparticles for colorimetric detection of biothiols by smartphone

Zijie Wei, Cuifeng Jiang, Xinyan Chen, Hui Li, and Juan Song

School of Materials Science and Engineering, Yancheng Institute of Technology, Yancheng, China



Received: 11 December 2023  Accepted: 25 February 2024


In this study, Os/Ag nanoparticles (Os/Ag NPs) with smaller Km and almost five times Vmax compared to Os NPs were prepared. This not only reduced the cost of nanozyme, but also improved the catalytic ability. Addition of L-Cysteine or glutathione (GSH) resulted in inhibitory effect on the peroxidase-like activity of Os/Ag nanozyme. Therefore, the L-Cysteine and GSH could be detected by absorption spectra method with limit of detection (LOD) of 0.22 μM and 0.2 μM, respectively. Moreover, a colorimetric sensing system for L-Cysteine and GSH based on portable paper strip and smartphone was fabricated with LOD of 1.72 μM and 1.4 μM, respectively. Furthermore, this method can be used in fetal bovine serum with recovery rates ranging from 89.42% to 109.0%. The sensing system exhibited good selectivity. This proposed colorimetric sensing assay holds great potential application in the detection of biothiols when no sophisticated equipment are available.

Graphical abstract

Scheme illustration for the colorimetric detection of L-Cysteine and GSH by use of smartphone

Keywords: Os/Ag NPs; Nanozyme; Peroxidase-like activity; Biothiol; Smartphone

Full paper is available at

DOI: 10.1007/s11696-024-03391-9


Chemical Papers 78 (7) 4295–4305 (2024)

Thursday, June 13, 2024

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