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Purification with macroporous resin and antioxidant activity of polyphenols from sweet potato leaves

Li-Feng Yang, Wei Nie, Yan-Ping Cui, Feng-Li Yue, Xiao-Tong Fan, and Ru-Ying Sun

College of Food Science and Engineering, Shandong Agriculture and Engineering University, Jinan, China

 

E-mail: z2015003@sdaeu.edu.cn

Received: 21 January 2023  Accepted: 31 July 2023

Abstract:

In this study, polyphenols from sweet potato leaves were purified by macroporous resin, and antioxidant activity was evaluated by free radical scavenging. AB-8, D-101, HPD-100 and DM301 four resins were selected for purification. The antioxidant activity was evaluated by 2,4-dinitrophenylhydrazine (DPPH) and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) methods. The results showed that the DM-301 macroporous adsorption resin was most suitable by static adsorption–desorption method, and the optimal purification conditions were determined by single factor experiment as follows: the concentration of sample solution 6.0 mg/mL, the flow rate of sample solution 60 mL/min, the volume fraction of ethanol desorption 60%, and the flow rate 40 mL/h. The scavenging rates of DPPH and ABTS free radicals by purified polyphenols from sweet potato leaves were 74.04% and 98.20%, respectively. The research results provide reference for further development and utilization of polyphenols from sweet potato leaves.

Keywords: Polyphenols; Macroporous resin; Purify; Antioxidant activity

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-023-03058-x

 

Chemical Papers 78 (1) 181–188 (2024)

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