ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7

Published monthly

Chemiluminescence of horseradish peroxidase in water–ionic liquid microemulsion: an approach from catalytic point of view

Seyed Mohammad Abedirad and Mojtaba Shamsipur

Department of Chemistry, Razi University, Kermanshah, Iran



Received: 28 November 2022  Accepted: 5 April 2023


Microemulsions of ionic liquids (ILs) are added to enhance the activity of peroxidase. From a catalytic standpoint, we provide the first research of the chemiluminescence (CL) features of luminol microencapsulated with horseradish peroxidase (HRP) in water–ionic liquid (W/IL) microemulsions. Water/AOT (sodium bis (2-ethyl-1-hexyl) sulfosuccinate)/hydrophobic IL [C8mim] [PF6] (1-octyl-3-methylimidazolium hexafluorophosphate)/1-hexanol constituted the system. Experiments demonstrated that the CL kinetic parameters, such as pseudo-first-order rise and fall rate constants for the chemiluminescence burst, CL intensity at time, maximum level intensity, total light yield, the intensity values at maximum CL, and time to reach maximum intensity, are dramatically affected as a function of W0 (water/surfactant), co-surfactant percentage, pH, and reactant concentrations. At W0 = 6, the maximum performance was achieved. The system exhibits more chemiluminescence properties than an enzymatic aqueous CL system and may be promoted without the need of an enhancer.

Graphical abstract

Keywords: Water–ionic liquid microemulsion; Enzymatic chemiluminescence; Horseradish peroxidase; Kinetic parameters

Full paper is available at

DOI: 10.1007/s11696-023-02815-2


Chemical Papers 77 (8) 4659–4669 (2023)

Tuesday, May 28, 2024

SCImago Journal Rank 2021
European Symposium on Analytical Spectrometry ESAS 2022
© 2024 Chemical Papers