ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Curcumin–hydroxypropyl-\(\beta\)-cyclodextrin complex preparation methods: a comparative study

Bably Khatun, Muzamil Ahmad Rather, Shubham Rohilla, Raveena Borah, Manabendra Mandal, and T. K. Maji

Department of Chemical Sciences, Tezpur University, Napaam, Tezpur, India

E-mail: bablynano@gmail.com

Received: 4 September 2022  Accepted: 14 March 2023

Abstract:

The failure of curcumin as a full-fledged drug is predominant because of its less bioavailability. This limitation can be overcome by forming an inclusion complex with HP\(\beta\)CD. There are different methodologies reported in the literature for the preparation of inclusion complexes, yet a detailed comparative study to choose the appropriate method has not been reported to the best of our knowledge. This study provides a detailed comparative study of five different methods [co-evaporation (COE), co-precipitation (CPT), solvent evaporation (SE), Kneading (KD) and incubator shaking (IS)] of curcumin–HP\(\beta\)CD inclusion complex formation and their evaluation. The complexes were prepared and characterized by different analytical techniques like Fourier Transform Infrared Spectroscopy, Thermogravimetric analysis, Differential Scanning Calorimetry, X-Ray diffractometry, Scanning Electron Microscopy and Transmission Electron Microscopy. All the complexes exhibited better performances compared to curcumin. The loading and encapsulation efficiencies of KD were more followed by CPT, COE, SE and IE; nevertheless, the values of SE and IS were significantly less. The aqueous solubilities of the complexes followed the trend IS>SE>COE>KD>CPT. The swelling of and hence, curcumin release from COE was maximum followed by KD, SE, IS, and CPT. However, CPT demonstrated the best glucose uptake, antioxidant and anticancer activities followed by COE and KD. COE exhibited better antibacterial activities than KD and CPT, but the difference between COE and KD is not significant. Despite demonstrating various bioactivities, the samples were found non-toxic to human RBCs.

Keywords: Curcumin; Inclusion complex; HP\(\beta\)CD; Nanoparticles

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-023-02791-7

Chemical Papers 77 (8) 4409–4424 (2023)