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Interaction of tacrine-coumarin derivatives with double stranded DNA: spectroscopic and molecular docking study

Eva Konkoľová, Monika Hudáčová, Slávka Hamuľaková, Katarzyna E. Nowak, and Mária Kožurková

Department of Biochemistry, Institute of Chemistry, Faculty of Science, Pavol Jozef Šafárik University in Košice, Košice, Slovak Republic

 

E-mail: konkolova.eva1@gmail.com

Received: 30 November 2022  Accepted: 26 February 2023

Abstract:

Identifying the ways in which drugs interact with DNA is crucial from a pharmacological point of view, and therefore determining the binding mode of tacrine-coumarin derivatives with DNA is key to gaining a fuller understanding of the activity of these compounds. Seven tacrine-coumarin derivatives 1a1d and 2a2c have been evaluated for their binding affinity to calf thymus DNA (ctDNA) by a variety of spectroscopic techniques including UV–Vis absorbance, fluorescence and CD spectroscopy. The effect of 1a1d and 2a2c on thermal denaturation profiles of ctDNA was also studied. The binding constants for the ctDNA-drug complexes were determined on the basis of spectrophotometric and spectrofluorometric titrations. Evidence has been provided that the tacrine-coumarin hybrid molecules bind to ctDNA through a non-intercalative mode of interaction such as groove binding. For derivatives 1d and 2c molecular docking was performed for two different DNA conformations. The results of preliminary molecular docking show that the most favourable DNA binding mode of these derivatives is non-intercalative.

Keywords: Tacrine-coumarin derivatives; DNA interaction; DNA binding mode; Molecular docking study

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-023-02759-7

 

Chemical Papers 77 (7) 3993–4006 (2023)

Wednesday, July 24, 2024

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