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Spectrofluorimetric assay of amlodipine and perindopril in their raw materials, pharmaceutical formulations and spiked human plasma through the formation of complexes with Eosin Y

Hesham Salem, Amany Abdelaziz, Sayed Derayea, Dalia M. Nagy, and Khalid M. Badr El-Din

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, Egypt



Received: 24 September 2022  Accepted: 2 January 2023


For quantitation of amlodipine (AML) and perindopril (PER) in their authentic, pharmaceutical formulations and spiked human plasma, a simple, sensitive, validated and inexpensive spectrofluorimetric method has been developed. The proposed method is developed to be based on quantitative quenching effect of two antihypertensive drugs on Eosin Y's native fluorescence which was achieved by developing binary complexes between each of the cited drugs in an acidic environment using acetate buffer (pH 4.4) with Eosin Y. Fluorescence quenching was recorded at 544 nm after excitation at 425 nm. For AML and PER, calibration curves were obtained over the range of 0.3–3.0 µg/mL and 0.2–2.0 µg/mL, respectively, with correlation coefficients of 0.9993 and 0.9995, respectively. The developed method was validated according to ICH guidelines. The proposed spectrofluorimetric method is regarded new and sensitive. As a result, the proposed method might be used to estimate the quality of the cited drugs in their pharmaceutical formulations and biological fluid.

Keywords: Amlodipine; Perindopril; Eosin Y; Spectrofluorimetric assay; Quenching effect

Full paper is available at

DOI: 10.1007/s11696-023-02668-9


Chemical Papers 77 (5) 2817–2825 (2023)

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