ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7

Published monthly

Spiropyran 5,6-dichloro-1,3,3-trimethylspiro[indoline-2,2-2H-pyrano[3,2-h]quinoline] application as a spectorphotometric and fluorescent probe for glutathione and cysteine sensing

A. A. Shcherbatykh, M. S. Chernov’yants, N. A. Voloshin, and A. V. Chernyshev

Department of Chemistry, Southern Federal University, Rostov-on-Don, Russia



Received: 16 November 2021  Accepted: 1 May 2022


Sulfur-containing amino acids play an essential role in the physiological process. Spiropyrans are a class of photochromic substances, which can be used for the determination of sulfur-containing amino acids and oligopeptides, such as cysteine, methionine, glutathione. Herein we used spiropyran 5,6′-dichloro-1,3,3-trimethylspiro[indoline-2,2′-2H-pyrano[3,2-h]quinoline] (DTIQ) for analytes sensing. DTIQ demonstrates a selective response toward glutathione in the presence of cysteine and methionine. It allows us to develop simple, rapid and sensible methods of spectrophotometric and fluorimetric glutathione assay. The developed methods were applied to quantification of glutathione in the dietary supplement and human urine. At the same time, DTIQ was found to bind Cu2+ with the formation of complex, which can be destroyed by glutathione or cysteine. Since that is accompanied by absorption changes, DTIQ application as a glutathione and cysteine “ON–OFF-ON” sensor is possible. The elaborated methods are characterized by low limits of detection, which are quite enough to quantify analytes at the level of their physiological concentration.

Keywords: Cysteine; Glutathione; Spiropyrans; Spectrophotometry; Fluorimetry

Full paper is available at

DOI: 10.1007/s11696-022-02259-0


Chemical Papers 76 (9) 5541–5550 (2022)

Sunday, June 23, 2024

SCImago Journal Rank 2021
European Symposium on Analytical Spectrometry ESAS 2022
© 2024 Chemical Papers