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A comparative study of biomimetic fluoroimmuno-assays based on 2,4-D imprinted polymers prepared by different polymerization techniques

Amina Hoji, Turghun Muhammad, Muyasier Wubulikasimu, Mukhtar Imerhasan, and Ayzukram Yasen

State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources; College of Chemistry, Xinjiang University, Urumqi, People’s Republic of China

 

E-mail: turghunm@xju.edu.cn

Received: 2 October 2021  Accepted: 18 February 2022

Abstract:

Molecularly imprinted polymers (MIPs) are widely used in immunoassays as biomimetic receptors. MIPs are prepared by different polymerization methods and result in diverse performance in these assays. In this paper, MIPs were prepared via bulk polymerization, precipitation polymerization and a surface polymerization, which is newly developed by our group, to compare their performances in a biomimetic fluoroimmunosorbent assays. The imprinted polymers were prepared using the herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D) as the template, 4-vinylpyndine and ethylene glycol dimethacrylate as the functional and crosslinking monomer respectively by thermal polymerization. A novel fluorescent derivative of 2,4-D was synthesized from 9-anthracenemethanol to compete the binding sites of the MIPs with 2,4-D. The results show that the polymer coating provides the largest concentration range and the most facile format for the competitive immunoassay. The sandwich method can be used to produce very stable and reusable polymer coating which is expected to replace polymer particles in immunoassays.

Keywords: Molecularly imprinted polymers; Biomimetic immunoassay; Fluorescent derivative; Polymer coating; 2,4-D

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02142-y

 

Chemical Papers 76 (6) 3963–3970 (2022)

Friday, May 24, 2024

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