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A solvent collection technique using dispersive liquid–liquid microextraction coupled with spectrophotometry for the trace determination of folic acid in pure, dosage forms and flaxseed

Sally Ghanem Ahmed and Wijdan Shakir Khayoon

Department of Chemistry, College of Sciences, University of Baghdad, Baghdad, Iraq



Received: 22 August 2021  Accepted: 16 December 2021


A simple, rapid and environmentally friendly dispersive liquid–liquid microextraction method-based spectrophotometric method for the trace determination of folic acid has been developed. The proposed method is based on the formation of a deep yellow product via reaction of folic acid and 1,2-naphthoquine-4-sulfonate at pH = 9. The formed complex was extracted using a mixture of chloroform and ethanol. Then, the tiny organic droplets were measured at λ = 520 nm. At the optimum conditions, linearity was ranged from 0.05 to 1.5 μg/mL for the standard and samples, with a linear correlation coefficient of 0.9996. The detection limits were 0.02, 0.027, 0.03, 0.02 and 0.04 μg/mL for standard, tablet (5 mg), tablet (1 mg), syrup and flaxseed, respectively, and limits of quantification were 0.066, 0.089, 0.099, 0.066 and 0.132 μg/mL for standard, tablet (5 mg), tablet (1 mg), syrup and flaxseed, respectively. Good recoveries and enrichment factor ranged 94.6–103.8% and 20–27, respectively, for the pharmaceutical and flaxseed samples were obtained. The method was successfully applied for the determination of folic acid in pure, dosage forms and flaxseed.

Keywords: Folic acid; Spectrophotometry; 1-2-naphthoquine-4-sulfonate; Pharmaceuticals preparations; Dispersive liquid–liquid microextraction; Flaxseed; Vitamin B9

Full paper is available at

DOI: 10.1007/s11696-021-02027-6


Chemical Papers 76 (4) 2485–2494 (2022)

Tuesday, May 21, 2024

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