ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Three-dimensional magnetic enzyme-inorganic hybrid nanocomplexes with high reusability and stability to obtain lactose-free products

Fei Yi, Yu-Cheng Liu, Yun-Jia Yang, Xing-Hu Ji, and Zhi-Ke He

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People’s Republic of China

E-mail: zhkhe@whu.edu.cn

Received: 4 April 2021  Accepted: 1 June 2021

Abstract:

Multitudinous people suffer from lactose intolerance which is bound up with lactose malabsorption. Enzymatic hydrolysis is the most common approach for the preparation of lactose-free products. The recycling of hydrolytic enzymes is an effective way to produce low-cost lactose-free products. Herein, a facile strategy for the preparation of the novel Fe₃O₄ magnetic nanoparticles-β-galactosidase-CaHPO₄ hybrid nanocomplexes (β-gal MNCs) was established. β-gal MNCs were composed of amino-modified Fe₃O₄ magnetic nanoparticles, β-galactosidase and crystalline calcium hydrogen phosphates. Meanwhile, under the external magnetic field, the magnetic β-gal MNCs can be easily and rapidly separated from the reaction medium. In addition, the β-galactosidase maintained most of its initial activity after immobilization into the β-gal MNCs. In a continuous hydrolysis reaction, β-gal MNCs exhibited excellent reusability and good stability. Therefore, β-gal MNCs hold promising prospects in the fast and low-cost preparation of lactose-free milk.

Keywords: Magnetic β-galactosidase hybrid nanocomplexes; Magnetic separation; Enzyme reaction; Immobilization; Lactose hydrolysis

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-021-01726-4

Chemical Papers 75 (10) 5353–5362 (2021)