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Feasible study on poly(Pyrrole-co-Pyrrole-3-Carboxylic Acid)-modified electrode for detection of 17β-Estradiol

Normazida Rozi, Sharina Abu Hanifah, Mohd Hazani Mat Zaid, Nurul Huda Abd Karim, and Masato Ikeda

Department of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia



Received: 3 April 2020  Accepted: 9 March 2021


Preparation and characterizations of poly(pyrrole-co-pyrrole-3-carboxylic acid) (PPYPA) film on the surface of screen printed carbon electrode (SPE) has been discussed in the manuscript. Different ratios of monomer for PPYPA preparation will be applied to determine 17β-estradiol using cyclic voltammetry. The SPE was modified with PPYPA film using an electrodeposition method and followed by the immobilization of 75mer aptamer specifically binding to 17β-estradiol as a model study. The materials were characterized by cyclic voltammetry, Fourier transform infrared, electrochemical impedance spectroscopy and scanning electron microscopy. The redox peak potential was found to be at 0.13 V and 0.26 V. The appearance of cauliflower structure in the SEM image which indicates PPYPA film was formed on SPE. Moreover, PPYPA/SPE exhibited a lower charge transfer resistance (Rct) compared to bare SPE indicates a good conductivity of film. Our study shows that 3:1 ratio between PPY and pyrrole-3-carboxylic acid provided highly active surface area for the immobilization of 75mer aptamer for 17β-estradiol detection.

Keywords: Pyrrole; Pyrrole-3-carboxylic acid; 17β-estradiol; Electrochemical characterization

Full paper is available at

DOI: 10.1007/s11696-021-01597-9


Chemical Papers 75 (7) 3493–3503 (2021)

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