ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
A red-emitting fluorescent probe for visualizing mitochondrial microviscosity by cell imaging
Yeming Zhang, Bingsong Xu, Hao Chen, Bin Fang, Hui Wang, and Lei Hu
College of Pharmacy, Wannan Medical College, Wuhu, China
Received: 1 September 2020 Accepted: 7 January 2021
The visual detection of mitochondrial viscosity is of considerable significance in that it is associated with various cellular functions. Herein, a novel red-emitting viscosity probe YD-Mito constructed by linking phenylamine derivatives and benzo[e]indolium through a C–C double bond has been designed and synthesized. It was found that YD-Mito exhibited a special response to viscosity accompanying with a sharp fluorescence enhancement (20-fold) at 620 nm. Importantly, YD-Mito can precisely localized in mitochondria independent from membrane potential and visualized the mitochondrial viscosity. Therefore, probe YD-Mito could provide a powerful tool for the determination of mitochondrial viscosity.
Keywords: Viscosity; Mitochondrial; Cell imaging; TICT
Full paper is available at www.springerlink.com.
Chemical Papers 75 (6) 2517–2523 (2021)