ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
An ellipsometric biosensor using aptamer for the detection of mercuric ions
Tuğçe Ertan and Mustafa Oğuzhan Caglayan
Chemical Engineering Department, Sivas Cumhuriyet University, Sivas, Turkey
Received: 27 April 2020 Accepted: 6 July 2020
An ellipsometric biosensor, to be used in the detection of mercuric ions that affect the vital organs of living organisms, was developed. In this context, as a diagnostic element of the developed ellipsometric biosensor, a nucleic acid-based aptamer that is highly selective for mercuric ions (Hg2+) was used. Two different aptamers, a long and straight sequence, and a hairpin-structured oligonucleotide sequence were chosen. The detection sensitivity obtained by both aptamers was 0.237°/μM and 0.211°/μM, respectively. The detection limit was 0.89 pM Hg2+and 187 nM Hg2+ for linear- and hairpin-type oligonucleotide, respectively.
Keywords: DNA aptamer; Ellipsometry; Biosensor; Mercuric ion
Full paper is available at www.springerlink.com.
Chemical Papers 75 (1) 89–97 (2021)