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A novel capillary electrophoresis method for the quantification of asenapine in pharmaceuticals using Box-Behnken design

Sona Aliyeva, Sakine Atila Karaca, Alper Uğur, Arın Gül Dal Poçan, and Duygu Yeniceli Uğur

Faculty of Pharmacy, Department of Analytical Chemistry, Anadolu University, Eskisehir, Turkey



Received: 13 February 2020  Accepted: 18 June 2020


A new capillary electrophoresis (CE) method was presented for the determination of asenapine, an atypical antipsychotic drug, in pharmaceuticals. Box-Behnken design, an experimental design method, was used to investigate the effects of run buffer pH, run buffer concentration and applied potential on the separation of asenapine and granisetron (IS). The optimum conditions were phosphate buffer (15 mM, pH: 3.1) with 25.7 kV voltage and 20 ℃ capillary temperature. The method was validated according to ICH guideline. A good linearity was obtained in the concentration range of 0.27–6.4 µg/mL with LOD and LOQ values of 0.07 and 0.24 μg/mL, respectively. The precision and accuracy of the method were satisfying with intra and interday recovery values of 97.8–100.8% and RSD less than 2%. The proposed CE method was applied to asenapine tablets successfully, for the first time.

Graphical abstract

Keywords: Asenapine; Capillary electrophoresis; Determination; Experimental design; Tablet analysis

Full paper is available at

DOI: 10.1007/s11696-020-01256-5


Chemical Papers 74 (12) 4443–4451 (2020)

Wednesday, June 19, 2024

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