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Electrochemical Determination of Nitric Oxide in Blood Samples

M. Rievaj, J. Lietava, and D. Bustin

Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, SK-812 37 Bratislava

 

E-mail: bustin@cvt.stuba.sk

Received: 2 September 2003

Abstract: A simple, sensitive, and specific electroanalytical method using a chemically modified ultramicroelectrode sensor was applied for nitric oxide determination in blood samples. The sensor fabricated by electrodeposition of p-type semiconducting polymeric films on a carbon fibre and Nafion deposition in the final step of its preparation was selective to NO. The Nafion coating eliminated the diffusion and adhesion of anionic species oxidizable at the microelectrode surface. One-electron oxidation current of differential pulse voltammetry peak was used for the quantitative measurement of NO in the samples. The method was applied to NO analysis in real blood samples.

Full paper in Portable Document Format: 585a306.pdf

 

Chemical Papers 58 (5) 306–310 (2004)

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