ISSN: 0366-6352 (print version)
ISSN: 1336-9075 (electronic version)
Springer's Journal No. 11696

 

Photosynthesis-inhibiting effects of 2-benzylsulphanylbenzimidazoles in spinach chloroplasts

Katarína Kráľová, František Šeršeň, Matúš Peško, Věra Klimešová, and Karel Waisser

Institute of Chemistry, Comenius University, SK-842 15 Bratislava, Slovakia

 

E-mail: kralova@fns.uniba.sk

Abstract: Inhibition of photosynthetic electron transport (PET) in spinach chloroplasts by nineteen 2-benzylsulphanylbenzimidazoles (BZA) was studied. BZA were found to inhibit photosynthetic electron transport (PET) and for their inhibitory efficiency, electronic properties of the R substituent on the benzyl moiety are decisive. The PET inhibiting activity of the studied BZA expressed as IC50 varied in the range from 28.5 μM (R = 3,5-(CF3)2) to 394.5 μM (R = 2,4-(NO2)2). For compounds with R = H, 4-CH3, 3-CH3, 3-OCH3, 4-F, 3-F, 4-Cl, 3-Cl, 2-Cl, 4-Br, 3-Br, 3,4-F2, 3,4-Cl2, 3-CF3, 3,5-(CF3)2 linear increase of the inhibitory activity with the increasing value of the substituent’s σ constant up to 0.86 was observed. Further increase of the σ constant resulted in a sharp activity decrease of the corresponding compounds (R = 2-F-6-Cl, 2-NO2, 3,5-(NO2)2, 2,4-(NO2)2). Using EPR spectroscopy and an artificial electron donor diphenyl carbazide it was found that the site of BZA action in the photosynthetic apparatus is situated on the donor side of PS 2, prior to the Z·/D· intermediate.

Keywords: alkylsulfanyl – benzimidazole – EPR spectroscopy – photosynthetic electron transport – site of action

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-012-0192-9

 

Chemical Papers 66 (8) 795–799 (2012)

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